The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 87 kDa.
Use a concentration of 3 µg/ml.
FunctionRequired for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
HIF1 beta antibody (ab54786) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human lung.
Western blot - HIF1 beta antibody (ab54786)
Predicted band size : 87 kDa HIF1 beta antibody (ab54786) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Immunocytochemistry/ Immunofluorescence - HIF1 beta antibody (ab54786)Image courtesy of an anonymous Abreview.
ab54786 staining HIF1 beta in human glioblastoma cell line U373MG by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 0.5% BSA/TBS/Tween for 20 minutes at room temperature. Samples were then incubated with primary antibody at 1/75 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Cy3® (red) used at a 1/400 dilution.