Fusion protein, containing amino acids 496-789 of Human HIF-1 beta.
Our Abpromise guarantee covers the use of ab2 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP/Chip||Use at an assay dependent concentration. PubMed: 20395365|
|IP||Use at an assay dependent concentration.|
|WB||1/2000. Predicted molecular weight: 92 kDa. Background non-specific bands are minimal on MCF7 human nuclear extracts. Background may increase with whole cell extract preparations.|
|ChIP||1/10 - 1/500.
A customer used in a ChIP assay after purifying this antibody and applyed 0.2 µg/µg chromatin. Please see Genpathway's abreview.
|ICC/IF||1/500. PubMed: 25422886|