The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Application notesIs unsuitable for WB.
FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
Detection of HIF1AN by Western Blot of Immunprecipitate.
An antibody to HIF1AN at 1µg/ml staining HIF1AN in 293T whole cell lysate immunoprecipitated using ab125199 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References for Anti-HIF1AN antibody (ab125199)
has not yet been referenced specifically in any publications.
Publishing research using ab125199? Please let us know so that we can cite the reference in this datasheet.
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