Anti-HIF1AN/FIH-1 antibody (ab187524)
Key features and details
- Rabbit polyclonal to HIF1AN/FIH-1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HIF1AN/FIH-1 antibody
See all HIF1AN/FIH-1 primary antibodies -
Description
Rabbit polyclonal to HIF1AN/FIH-1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human HIF1AN/FIH-1.
Database link: Q9NWT6 -
Positive control
- MCF7, K562, U937, 293T cell lysates; mouse heart;and skeletal muscle lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/50 - 1/200.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa. |
IHC-P
1/50 - 1/200. |
Target
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Function
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. -
Sequence similarities
Contains 1 JmjC domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Clone ID: BC007719 Human
- Entrez Gene: 55662 Human
- Entrez Gene: 319594 Mouse
- Omim: 606615 Human
- SwissProt: Q9NWT6 Human
- SwissProt: Q8BLR9 Mouse
- Unigene: 500788 Human
- Unigene: 145278 Mouse
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Alternative names
- DKFZp762F1811 antibody
- Factor inhibiting HIF-1 antibody
- Factor inhibiting HIF1 antibody
see all
Images
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All lanes : Anti-HIF1AN/FIH-1 antibody (ab187524) at 1/500 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : U937 lysate
Lane 4 : 293T cell lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse skeletal muscle lysate
Predicted band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling HIF1AN/FIH-1 with ab187524 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling HIF1AN/FIH-1 with ab187524 at 1/200. Magnification: 400x.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187524. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab187524 has been referenced in 9 publications.
- Yang W et al. TSP-1 as a novel biological marker of tumor vasculature normalization in colon carcinoma induced by Endostar. Oncol Lett 19:2107-2114 (2020). PubMed: 32194708
- Reddy SS et al. Effect of vitamin B12 supplementation on retinal lesions in diabetic rats. Mol Vis 26:311-325 (2020). PubMed: 32355441
- Pei B et al. IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1a signaling pathway. Biosci Rep 40:N/A (2020). PubMed: 32347300
- Mi L et al. HMGB1/RAGE pro-inflammatory axis promotes vascular endothelial cell apoptosis in limb ischemia/reperfusion injury. Biomed Pharmacother 116:109005 (2019). PubMed: 31136947
- Cai M et al. Hypoxia-induced mitochondrial translocation of DNM1L increases mitochondrial fission and triggers mPTP opening in HCC cells via activation of HK2. Oncol Rep 42:1125-1132 (2019). PubMed: 31322265
- Liu Y et al. Adipose-derived exosomes deliver miR-23a/b to regulate tumor growth in hepatocellular cancer by targeting the VHL/HIF axis. J Physiol Biochem 75:391-401 (2019). PubMed: 31321740
- Mei Z et al. a7-nAchR agonist GTS-21 reduces radiation-induced lung injury. Oncol Rep 40:2287-2297 (2018). PubMed: 30106431
- Johansson E et al. CD44 Interacts with HIF-2a to Modulate the Hypoxic Phenotype of Perinecrotic and Perivascular Glioma Cells. Cell Rep 20:1641-1653 (2017). WB ; Mouse . PubMed: 28813675
- Ren L et al. Hypoxia-induced CCL28 promotes recruitment of regulatory T cells and tumor growth in liver cancer. Oncotarget 7:75763-75773 (2016). WB ; Human . PubMed: 27716621