The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa).
FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
Lindholm ME et al. Negative regulation of HIF in skeletal muscle of elite endurance athletes: a tentative mechanism promoting oxidative metabolism. Am J Physiol Regul Integr Comp Physiol307:R248-55 (2014).
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Schwacha MG et al. Burn injury-induced alterations in wound inflammation and healing are associated with suppressed hypoxia inducible factor-1alpha expression. Mol Med14:628-33 (2008).
Read more (PubMed: 18615157) »