Overview

  • Product nameAnti-HIF1AN antibody
    See all HIF1AN primary antibodies
  • Description
    Rabbit polyclonal to HIF1AN
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide from within a region of internal sequence amino acids 287-336 (GITITVNFWY KGAPTPKRIE YPLKAHQKVA IMRNIEKMLG EALGNPQEVG) of Human HIF1AN (NP_060372)

  • Positive control
    • Human fetal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
  • Sequence similaritiesContains 1 JmjC domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp762F1811 antibody
    • Factor inhibiting HIF-1 antibody
    • Factor inhibiting HIF1 antibody
    • FIH 1 antibody
    • FIH-1 antibody
    • FIH1 antibody
    • FLJ20615 antibody
    • FLJ22027 antibody
    • HIF1AN antibody
    • HIF1N_HUMAN antibody
    • Hypoxia inducible factor 1 alpha inhibitor antibody
    • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
    • Hypoxia inducible factor asparagine hydroxylase antibody
    • Hypoxia-inducible factor 1-alpha inhibitor antibody
    • Hypoxia-inducible factor asparagine hydroxylase antibody
    • Peptide aspartate beta dioxygenase antibody
    see all

Anti-HIF1AN antibody images



  • Predicted band size : 40 kDa
    Western blot analysis of Human fetal heart tissue lysate labeling HIF1AN with ab86176 at 1.0µg/ml.
  • Anti-HIF1AN antibody (ab86176) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Gel concentration: 12%

References for Anti-HIF1AN antibody (ab86176)

ab86176 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"