The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/100.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/250 - 1/500.
WB: 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
References for Anti-HIF1AN antibody [EPR3658] (ab92498)
This product has been referenced in:
Ren L et al. Hypoxia-induced CCL28 promotes recruitment of regulatory T cells and tumor growth in liver cancer. Oncotarget7:75763-75773 (2016).
Read more (PubMed: 27716621) »
Bartoszewska S et al. The hypoxia-inducible miR-429 regulates hypoxia-inducible factor-1a expression in human endothelial cells through a negative feedback loop. FASEB J29:1467-79 (2015).
Read more (PubMed: 25550463) »