Overview

  • Product name
    Anti-HIF1AN antibody [EPR3658]
    See all HIF1AN primary antibodies
  • Description
    Rabbit monoclonal [EPR3658] to HIF1AN
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human HIF1AN.

  • Positive control
    • Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
ICC/IF
  • Application notes
    Flow Cyt: 1/100.
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/250 - 1/500.
    WB: 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
    • Sequence similarities
      Contains 1 JmjC domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp762F1811 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Anti-HIF1AN antibody [EPR3658] images

    • All lanes : Anti-HIF1AN antibody [EPR3658] (ab92498) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
    • ab92498 at 1/250 dilution staining HIF1AN in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92498 at 1/250 dilution staining HIF1AN in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92498 at 1/100 dilution staining HIF1AN in HeLa cells by Immunofluorescence.

    References for Anti-HIF1AN antibody [EPR3658] (ab92498)

    This product has been referenced in:
    • Ren L  et al. Hypoxia-induced CCL28 promotes recruitment of regulatory T cells and tumor growth in liver cancer. Oncotarget 7:75763-75773 (2016). WB ; Human . Read more (PubMed: 27716621) »
    • Bartoszewska S  et al. The hypoxia-inducible miR-429 regulates hypoxia-inducible factor-1a expression in human endothelial cells through a negative feedback loop. FASEB J 29:1467-79 (2015). Read more (PubMed: 25550463) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab92498.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up