The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: 1/100.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/250 - 1/500.
WB: 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
Is unsuitable for IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
ab92498 at 1/100 dilution staining HIF1AN in HeLa cells by Immunofluorescence.
References for Anti-HIF1AN antibody [EPR3658] (ab92498)
This product has been referenced in:
Chen T et al. Factor inhibiting HIF1a (FIH-1) functions as a tumor suppressor in human colorectal cancer by repressing HIF1a pathway. Cancer Biol Ther16:244-52 (2015).
Read more (PubMed: 25602156) »
Wang E et al. The role of factor inhibiting HIF (FIH-1) in inhibiting HIF-1 transcriptional activity in glioblastoma multiforme. PLoS One9:e86102 (2014).
Read more (PubMed: 24465898) »