Overview

  • Product nameAnti-HIF1AN antibody [EPR3658]
    See all HIF1AN primary antibodies
  • Description
    Rabbit monoclonal [EPR3658] to HIF1AN
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human HIF1AN.

  • Positive control
    • Jurkat, 293T and A375 cell lysates; Human breast carcinoma and brain tissue; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab92498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
Flow Cyt
ICC/IF
  • Application notesFlow Cyt: 1/100.
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/250 - 1/500.
    WB: 1/5000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionHydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases.
    • Sequence similaritiesContains 1 JmjC domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp762F1811 antibody
      • Factor inhibiting HIF-1 antibody
      • Factor inhibiting HIF1 antibody
      • FIH 1 antibody
      • FIH-1 antibody
      • FIH1 antibody
      • FLJ20615 antibody
      • FLJ22027 antibody
      • HIF1AN antibody
      • HIF1N_HUMAN antibody
      • Hypoxia inducible factor 1 alpha inhibitor antibody
      • Hypoxia inducible factor 1 alpha subunit inhibitor antibody
      • Hypoxia inducible factor asparagine hydroxylase antibody
      • Hypoxia-inducible factor 1-alpha inhibitor antibody
      • Hypoxia-inducible factor asparagine hydroxylase antibody
      • Peptide aspartate beta dioxygenase antibody
      see all

    Anti-HIF1AN antibody [EPR3658] images

    • All lanes : Anti-HIF1AN antibody [EPR3658] (ab92498) at 1/5000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A375 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
    • ab92498 at 1/250 dilution staining HIF1AN in Human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92498 at 1/250 dilution staining HIF1AN in Human brain by Immunohistochemistry, Paraffin-embedded tissue.
    • ab92498 at 1/100 dilution staining HIF1AN in HeLa cells by Immunofluorescence.

    References for Anti-HIF1AN antibody [EPR3658] (ab92498)

    This product has been referenced in:
    • Chen T  et al. Factor inhibiting HIF1a (FIH-1) functions as a tumor suppressor in human colorectal cancer by repressing HIF1a pathway. Cancer Biol Ther 16:244-52 (2015). WB ; Human . Read more (PubMed: 25602156) »
    • Wang E  et al. The role of factor inhibiting HIF (FIH-1) in inhibiting HIF-1 transcriptional activity in glioblastoma multiforme. PLoS One 9:e86102 (2014). IHC ; Human . Read more (PubMed: 24465898) »

    See all 2 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab92498.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"