Overview

  • Product nameAnti-HIGD1A antibody
  • Description
    Rabbit polyclonal to HIGD1A
  • Specificityab117456 is predicted to not cross-react with HIG2.
  • Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to 19 amino acids near the N terminal of Human HIGD1A (NP_001093138).

  • Positive control
    • 293 cell lysate.
  • General notesAvoid repeated freeze thaw cycles and exposure to prolonged high temperatures.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab117456 was purified by affinity chromatography via a peptide column.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 2.5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.

Target

Anti-HIGD1A antibody images

  • Immunofluorescence of HIGD1A in 293 cells tissue with ab117456 at 10 ug/mL.
  • Lane 1 : Anti-HIGD1A antibody (ab117456) at 0.5 µg/ml
    Lane 2 : Anti-HIGD1A antibody (ab117456) at 1 µg/ml

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 12 kDa
  • Immunocytochemical analysis of 293 cells, staining HIGD1A with ab117456 at 2.5 µg/ml.

References for Anti-HIGD1A antibody (ab117456)

ab117456 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (C2C12, MEF)
Loading amount 25 µg
Specification C2C12, MEF
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2012

Application Western blot
Sample Human Cell lysate - whole cell (HepG2, 293T)
Loading amount 0 µg
Specification HepG2, 293T
Gel Running Conditions Reduced Denaturing (15% gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Aug 23 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"