Overview

  • Product name
    Anti-HIGD2B antibody
  • Description
    Rabbit polyclonal to HIGD2B
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MATLGFVTPE APFESSKPPI FEGLSPTVYS NPEGFKEKFL RKTRENP, corresponding to N terminal amino acids 1-47 of Human HIGD2B

  • Positive control
    • Human heart muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • Relevance
    Multipass membrane protein. Contains 1 HIG1 domain.
  • Cellular localization
    Cell Membrane.
  • Database links
  • Alternative names
    • HIG1 domain family member 2B pseudogene antibody
    • HIGD2BP antibody

Images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in cytoplasm. Recommended concentration of ab121586 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121586, at a 1/200 dilution, staining HIGD2BP in myocytes of paraffin-embedded Human heart muscle tissue by immunohistochemistry.

References

ab121586 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121586.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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