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Products:Immunology >> Innate Immunity >> Cytokines >> Interferons
Overview
Product name
High Sensitivity Interferon Gamma Human ELISA Kit (1 x 96 Well Plates)
See all Interferon gamma products (13) ...
Specificity
Recognizes both natural and recombinant human Interferon Gamma
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 3.9% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| Overall | 8.6% |
Tests
1 x 96 well plate
Sample type
Cell culture supernatant, Serum, Plasma
Assay type
Sandwich
Sensitivity
< 0.69 pg/ml
Range
0.78 pg/ml - 25 pg/ml
Recovery
| Sample type | Average % | Range % |
|---|---|---|
| Cell culture supernatant | 108 | - |
| Serum | 92 | - |
| Plasma | 72 | - |
Assay time
3h 00m
Cross reactivity
Reacts with
Human
Product overview
The Human high sensitivity IFN gamma ELISA is to be used for the in-vitro quantitative determination interferon gamma (IFN gamma )in human sera, plasmas, buffered solutions or cell culture media. The assay will recognize both natural and recombinant human IFN gamma. This kit is intended for research use only and is not to be used in diagnostic procedures.
Principle:
The IFN gamma Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for IFN gamma has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IFN gamma concentrations and unknowns are pipetted into these wells.
During the first incubation, the IFN gamma antigen and a biotinylated monoclonal antibody specific for IFN gamma are successively incubated.
After washing, the enzyme (streptavidin-peroxydase) is added. All the unbound enzyme is removed by washing and the first amplification step is performed by adding the Biotine-Tyramine reagent. Under the action of HRP, a biotine polymerisation reaction occurs in the region of the HRP linked to the detection antibody. After washing the second amplification step is performed and the polymerised biotine is revealed by a new streptavidin-HRP step. Finally after washing, the substrate is added. The intensity of this coloured product is directly proportional to the concentration of IFN gamma present in the samples.
Notes
Safety Information:
- For research use only.
- No human blood components included in this kit. Nevertheless, handling of human blood derivative samples should be in accordance with local safety procedures, e.g. CDC/NIH Health manual :
"Biosafety in Microbiological and Biomedical Laboratories 4th Edition" 1999 - Avoid any skin contact with H2SO4 and TMB. In case of contact, wash thoroughly.
- Do not eat, drink, smoke or apply cosmetics where kit reagents are used.
- Do not pipette with mouth.
- The stop solution in this kit contains 9.8% sulphuric acid.
Tested applications
ELISAmore details
Properties
Storage instructions
Store at 4°C. The kit is shipped at room temperature
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 12 strips | 8 units | |
| Amplification Diluent | Brown & blue spot | 1 x 25ml |
| Amplifier | Yellow | 1 x 200µl |
| Biotinylated Antibody Diluent | Red | 1 x 7.5ml |
| Biotinylated anti-IFN? | Red | 1 x 0.4ml |
| Chromogen TMB | Brown | 1 x 11ml |
| H2SO4: Stop Reagent | Black | 1 x 11ml |
| HRP Diluent | Red | 1 x 25ml |
| Standard | Yellow | 2 vials |
| Standard Diluent Buffer x10 Concentration | Black | 1 x 25ml |
| Streptavidin-HRP | 2 x 5µl | |
| Washing Buffer x200 Concentration | White | 1 x 10ml |
Research areas
Kits/ Lysates/ Other >> Kits >> ELISA and ELIPAIR Kits >> ELISA Kits >> Cytokines and cytokine receptors ELISA kits
Cancer >> Tumor immunology >> Cytokines >> Interferons
Stem Cells >> Signaling Pathways >> TGF beta >> Interferon gamma
Immunology >> Innate Immunity >> Cytokines >> Interferons
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ELISA - Human high sensitivity Interferon gamma ELISA Kit (ab46048)
(enlarge)
Relevance
Mammalian Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Alternative names
- IF 1
- IFG
- IFI
see all
Database links
- Entrez Gene: 3458 Human
- Omim: 147570 Human
- SwissProt: P01579 Human
Applications
Show applications key
Our Abpromise guarantee covers the use of ab46048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
High Sensitivity Interferon Gamma Human ELISA Kit (1 x 96 Well Plates) images:
ELISA - Human high sensitivity Interferon gamma ELISA Kit (ab46048)
Typical IFN gamma High sensitivity standard curve ranging from 0.78 to 25 pg/mL
References for High Sensitivity Interferon Gamma Human ELISA Kit (1 x 96 Well Plates) (ab46048)
ab46048 has not yet been referenced specifically in any publications.
Publishing research using ab46048? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"