Overview

  • Product nameAnti-HINT1 antibody
    See all HINT1 primary antibodies
  • Description
    Rabbit polyclonal to HINT1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HINT1.

    (Peptide available as ab71589.)

  • Positive control
    • This antibody gave a positive siganl in the following Human Lysates: HCT 116 Whole Cell, Jurkat Whole Cell, HEK293 Whole Cell, HepG2 Whole Cell, Colon Tissue Lysate, Kidney Tissue Lysate, Liver Tissue Lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/2000.
ICC/IF Use a concentration of 1 µg/ml.

Target

Anti-HINT1 antibody images

  • Anti-HINT1 antibody (ab64071) at 1 µg/ml + HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 15 minutes
  • ICC/IF image of ab64071 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64071, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, and MCF-7 cells at 5µg/ml.
  • All lanes : Anti-HINT1 antibody (ab64071) at 1 µg/ml

    Lane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Human colon tissue lysate - total protein (ab30051)
    Lane 6 : Human kidney tissue lysate - total protein (ab30203)
    Lane 7 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 14 kDa
    Observed band size : 14 kDa
    Additional bands at : 60 kDa,70 kDa,90 kDa. We are unsure as to the identity of these extra bands.
  • IHC-P image of Hint1 (ab64071) on Colon cancer sections. The sections were fixed in paraformaldehyde and underwent heat mediated antigen retrieval using Novacastra (pH6). The sections were then blocked in 0.3% H2O2 solution for 10 mins at 22°C.

    See Abreview

References for Anti-HINT1 antibody (ab64071)

ab64071 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Colon cancer)
Specification Colon cancer
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novacastra pH 6
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 0.3% · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

Submitted Oct 11 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"