Overview

  • Product name
  • Description
    Rabbit polyclonal to HINT1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 17 amino acid peptide from near the center of human HINT1 (NP_005331).

  • Positive control
    • Jurkat lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 14 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Belongs to the HINT family.
    Contains 1 HIT domain.
  • Domain
    The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
  • Cellular localization
    Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenosine 5' monophosphoramidase antibody
    • Adenosine 5''-monophosphoramidase antibody
    • HINT 1 antibody
    • HINT1 antibody
    • HINT1_HUMAN antibody
    • Histidine triad nucleotide binding protein 1 antibody
    • Histidine triad nucleotide-binding protein 1 antibody
    • PKCI 1 antibody
    • PKCI-1 antibody
    • PKCI1 antibody
    • PRKCNH1 antibody
    • Protein kinase C inhibitor 1 antibody
    • Protein kinase C interacting protein 1 antibody
    • Protein kinase C-interacting protein 1 antibody
    see all

Images

  • Lane 1 : Anti-HINT1 antibody (ab77086) at 1 µg/ml
    Lane 2 : Anti-HINT1 antibody (ab77086) at 2 µg/ml

    Lane 1 : Jurkat lysate
    Lane 2 : Jurkat lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

References

ab77086 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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