Recombinant
RabMAb

Anti-HINT1 antibody [EPR5109] (ab109319)

Overview

  • Product name
    Anti-HINT1 antibody [EPR5109]
    See all HINT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5109] to HINT1
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human HINT1.

  • Positive control
    • 293T, Jurkat, Raji, and HeLa cell lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the HINT family.
      Contains 1 HIT domain.
    • Domain
      The histidine triad, also called HIT motif, forms part of the binding loop for the alpha-phosphate of purine mononucleotide.
    • Cellular localization
      Cytoplasm. Nucleus. Interaction with CDK7 leads to a more nuclear localization.
    • Information by UniProt
    • Database links
    • Alternative names
      • Adenosine 5' monophosphoramidase antibody
      • Adenosine 5''-monophosphoramidase antibody
      • HINT 1 antibody
      • HINT1 antibody
      • HINT1_HUMAN antibody
      • Histidine triad nucleotide binding protein 1 antibody
      • Histidine triad nucleotide-binding protein 1 antibody
      • PKCI 1 antibody
      • PKCI-1 antibody
      • PKCI1 antibody
      • PRKCNH1 antibody
      • Protein kinase C inhibitor 1 antibody
      • Protein kinase C interacting protein 1 antibody
      • Protein kinase C-interacting protein 1 antibody
      see all

    Images

    • All lanes : Anti-HINT1 antibody [EPR5109] (ab109319) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa
    • ab109319 at 1/100 dilution staining HINT1 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

    References

    ab109319 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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