Overview

  • Product nameAnti-HINT3 antibody
  • Description
    Rabbit polyclonal to HINT3
  • Tested applicationsSuitable for: IP, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: MAEEQVNRSA GLAPDCEASA TAETTVSSVG TCEAAAKSPE PKDYDSTCVF CRIAGRQDPG TELLHCENED LICFKDIKPA ATHHYLVVP, corresponding to amino acids 1-89 of Human HINT3 (UniProt Q9NQE9).

  • Positive control
    • WB: RT4, U-251 MG, Human liver and Human tonsil lysates IHC-P: Human colon tissue ICC/IF: U-2 OS cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval via the pressure cooker method.
WB 1/250 - 1/500.
ICC/IF Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100

Target

  • FunctionHydrolyzes phosphoramidate and acyl-adenylate substrates.
  • Sequence similaritiesBelongs to the HINT family.
    Contains 1 HIT domain.
  • Cellular localizationCytoplasm. Nucleus. Localized as aggregates in the cytoplasm and the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HINT-3 antibody
    • HINT3 antibody
    • HINT3_HUMAN antibody
    • Histidine triad nucleotide-binding protein 3 antibody

Anti-HINT3 antibody images

  • All lanes : Anti-HINT3 antibody (ab121960) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab121960, at a 1/100 dilution, staining HINT3 in paraffin emebedded Human colon tissue by Immunohistochemistry.
  • ab121960, at 4 µg/ml, staining HINT3 in Human cell line U-2 OS by Immunofluorescence.

References for Anti-HINT3 antibody (ab121960)

ab121960 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Sample Escherichia coli Cell lysate - whole cell (E.coli cell lysate)
Total protein in input 2000 µg
Specification E.coli cell lysate
Immuno-precipitation step Other - Dynabeads
Username

Abcam user community

Verified customer

Submitted Sep 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"