Overview

  • Product name
  • Description
    Rabbit polyclonal to HIP1R
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HIP1R.

    (Peptide available as ab86622.)

  • Positive control
    • This antibody gave a positive signal in Human Brain Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 119 kDa (predicted molecular weight: 119 kDa).

Target

  • Function
    Component of clathrin-coated pits and vesicles, that may link the endocytic machinery to the actin cytoskeleton. Binds 3-phosphoinositides (via ENTH domain). May act through the ENTH domain to promote cell survival by stabilizing receptor tyrosine kinases following ligand-induced endocytosis.
  • Tissue specificity
    Brain, heart, kidney, pancreas, and liver, but not in lung or placenta.
  • Sequence similarities
    Belongs to the SLA2 family.
    Contains 1 ENTH (epsin N-terminal homology) domain.
    Contains 1 I/LWEQ domain.
  • Domain
    Binds F-actin via the talin-like I/LWEQ domain.
  • Cellular localization
    Cytoplasm > perinuclear region. Endomembrane system. Cytoplasmic vesicle > clathrin-coated vesicle membrane. Membrane-associated protein, mainly localized at the endocytic compartments and in the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ14000 antibody
    • FLJ27022 antibody
    • HIP-12 antibody
    • Hip1 related antibody
    • HIP1-related protein antibody
    • HIP12 antibody
    • HIP1R antibody
    • HIP1R_HUMAN antibody
    • HIP3 antibody
    • Huntingtin Interacting Protein 1 Related antibody
    • Huntingtin interacting protein 12 antibody
    • Huntingtin-interacting protein 1-related protein antibody
    • Huntingtin-interacting protein 12 antibody
    • ILWEQ antibody
    • KIAA0655 antibody
    • MGC47513 antibody
    see all

Images

  • Anti-HIP1R antibody (ab77297) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 119 kDa
    Observed band size : 119 kDa


    Exposure time : 5 minutes
  • ICC/IF image of ab77297 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77297, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 5µg/ml.
  • Xenopus laevis cytoplasmic egg extract visualized live with primary and secondary antibody addition [red is anti-Clathrin X22 (ab2731) with goat anti-mouse Alexa Fluor 568 secondary, green is anti-HIP1R (ab77297) with goat anti-rabbit Alexa Fluor 488 secondary]. HIP1R staining is specific for perinuclear membrane compartments.

    See Abreview

References

ab77297 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (Xenopus laevis cytoplasmic egg extract)
Specification
Xenopus laevis cytoplasmic egg extract
Fixative
live, no fixative
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Abreviews
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount
40 µg
Specification
Xenopus laevis cytoplasmic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 17 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up