Overview

  • Product nameAnti-HIP2 antibody
    See all HIP2 primary antibodies
  • Description
    Goat polyclonal to HIP2
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Chicken, Human
    Predicted to work with: Mouse, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-SWDVETATELLLSN

    , corresponding to C terminal amino acids 187-200 of Human HIP2.

  • Positive control
    • Jurkat and Human Liver lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab37917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 1 - 2 µg/ml.

Target

  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • Tissue specificityExpressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • Post-translational
    modifications
    Sumoylation at Lys-14 impairs catalytic activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 25K antibody
    • E2(25K) antibody
    • HIP-2 antibody
    • Huntingtin interacting protein 2 antibody
    • Huntingtin-interacting protein 2 antibody
    • HYPG antibody
    • LIG antibody
    • UBC1 antibody
    • UBE2K antibody
    • UBE2K_HUMAN antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 25 kDa antibody
    • ubiquitin conjugating enzyme E2K antibody
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin-conjugating enzyme E2 K antibody
    • Ubiquitin-conjugating enzyme E2(25K) antibody
    • Ubiquitin-conjugating enzyme E2-25 kDa antibody
    • Ubiquitin-conjugating enzyme E2-25K antibody
    • Ubiquitin-protein ligase antibody
    see all

Anti-HIP2 antibody images

  • Anti-HIP2 antibody (ab37917) at 0.5 µg/ml + Jurkat lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size : 23 kDa
  • Anti-HIP2 antibody (ab37917) at 1/1000 dilution + Cell lysate prepared from chicken DT40 lymphoblast cell line at 30 µg

    Secondary
    Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • ab37917 at 3.7 µg/ml staining HIP2 in Human cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-HIP2 antibody (ab37917)

ab37917 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Chicken Cell lysate - other (Soluble Fraction)
Loading amount 30 µg
Specification Soluble Fraction
Gel Running Conditions Reduced Denaturing (12.5-15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 25 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (Soluble Fraction)
Loading amount 30 µg
Specification Soluble Fraction
Gel Running Conditions Reduced Denaturing (12.5-15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted May 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"