• Product nameAnti-HIP2 antibody
    See all HIP2 primary antibodies
  • Description
    Rabbit polyclonal to HIP2
  • SpecificityA region of Human UBE2K
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (FTELRGEIAG PPDTPYEGGR YQLEIKIPET YPFNPPKVRF ITKIWHPNIS) of human HIP2 (NP_005330).

  • Positive control
    • HepG2 cell lysate


Associated products


Our Abpromise guarantee covers the use of ab82950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.


  • FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
  • Tissue specificityExpressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
    Contains 1 UBA domain.
  • Post-translational
    Sumoylation at Lys-14 impairs catalytic activity.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • E2 25K antibody
    • E2(25K) antibody
    • HIP-2 antibody
    • Huntingtin interacting protein 2 antibody
    • Huntingtin-interacting protein 2 antibody
    • HYPG antibody
    • LIG antibody
    • UBC1 antibody
    • UBE2K antibody
    • UBE2K_HUMAN antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin conjugating enzyme E2 25 kDa antibody
    • ubiquitin conjugating enzyme E2K antibody
    • ubiquitin conjugating enzyme E2K (UBC1 homolog, yeast) antibody
    • Ubiquitin protein ligase antibody
    • Ubiquitin-conjugating enzyme E2 K antibody
    • Ubiquitin-conjugating enzyme E2(25K) antibody
    • Ubiquitin-conjugating enzyme E2-25 kDa antibody
    • Ubiquitin-conjugating enzyme E2-25K antibody
    • Ubiquitin-protein ligase antibody
    see all

Anti-HIP2 antibody images

  • Anti-HIP2 antibody (ab82950) at 1 µg/ml (in 5% skim milk / PBS) + HepG2 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-HIP2 antibody (ab82950)

This product has been referenced in:
  • Fromm-Dornieden C  et al. Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells. BMC Mol Biol 13:9 (2012). Read more (PubMed: 22436005) »

See 1 Publication for this product

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