The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 22450813
Use at an assay dependent concentration.
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa).
FunctionActin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
Sequence similaritiesBelongs to the ABP1 family. Contains 1 ADF-H domain. Contains 1 SH3 domain.
DomainThe SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
Post-translational modificationsDegraded by caspases during apoptosis.
Hepper I et al. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol188:4590-601 (2012).
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