Overview

  • Product name
  • Description
    Rabbit polyclonal to HIP55
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human HIP55.

    (Peptide available as ab91635.)

  • Positive control
    • This antibody gave a positive signal in the following human whole cell lysates: SHSY-5Y; SK N SH; SK N BE; HeLa.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notes
    ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
    • Sequence similarities
      Belongs to the ABP1 family.
      Contains 1 ADF-H domain.
      Contains 1 SH3 domain.
    • Domain
      The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
    • Post-translational
      modifications
      Degraded by caspases during apoptosis.
    • Cellular localization
      Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
    • Information by UniProt
    • Database links
    • Alternative names
      • ABP1 antibody
      • Actin-binding protein 1 antibody
      • Cervical mucin associated protein antibody
      • Cervical mucin-associated protein antibody
      • Cervical SH3P7 antibody
      • CMAP antibody
      • Dbnl antibody
      • DBNL_HUMAN antibody
      • Drebrin F antibody
      • drebrin like antibody
      • Drebrin like protein antibody
      • Drebrin-F antibody
      • Drebrin-like protein antibody
      • HIP 55 antibody
      • HIP-55 antibody
      • HPK1 interacting protein of 55 kDa antibody
      • HPK1-interacting protein of 55 kDa antibody
      • SH3 domain containing protein 7 antibody
      • SH3 domain-containing protein 7 antibody
      • SH3P7 antibody
      • src homology 3 domain containing protein HIP 55 antibody
      see all

    Images

    • All lanes : Anti-HIP55 antibody (ab77147) at 1 µg/ml

      Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
      Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 48 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 48 kDa,72 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 5 minutes
    • ICC/IF image of ab77147 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

    References

    ab77147 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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