Overview

  • Product name
    Anti-HIP55 antibody [M334]
    See all HIP55 primary antibodies
  • Description
    Mouse monoclonal [M334] to HIP55
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to the C-terminal region of Human HIP55

  • Positive control
    • Jurkat, HeLa or A431 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    M334
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa.
ELISA 1/2000.

Target

  • Function
    Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
  • Sequence similarities
    Belongs to the ABP1 family.
    Contains 1 ADF-H domain.
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
  • Post-translational
    modifications
    Degraded by caspases during apoptosis.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABP1 antibody
    • Actin-binding protein 1 antibody
    • Cervical mucin associated protein antibody
    • Cervical mucin-associated protein antibody
    • Cervical SH3P7 antibody
    • CMAP antibody
    • Dbnl antibody
    • DBNL_HUMAN antibody
    • Drebrin F antibody
    • drebrin like antibody
    • Drebrin like protein antibody
    • Drebrin-F antibody
    • Drebrin-like protein antibody
    • HIP 55 antibody
    • HIP-55 antibody
    • HPK1 interacting protein of 55 kDa antibody
    • HPK1-interacting protein of 55 kDa antibody
    • SH3 domain containing protein 7 antibody
    • SH3 domain-containing protein 7 antibody
    • SH3P7 antibody
    • src homology 3 domain containing protein HIP 55 antibody
    see all

Images

  • All lanes : Anti-HIP55 antibody [M334] (ab86708) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : A431 cell lysate


    Observed band size : 55 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Fish L  et al. Muscleblind-like 1 suppresses breast cancer metastatic colonization and stabilizes metastasis suppressor transcripts. Genes Dev 30:386-98 (2016). Read more (PubMed: 26883358) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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