1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/100 - 1/1000. Fixation in ice cold methanol is recommended.
Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
Belongs to the ABP1 family. Contains 1 ADF-H domain. Contains 1 SH3 domain.
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
Immunofluorescent analysis of HeLa cells (fixed in iced-cold Methanol for 5 min) labeling HIP55 with ab154016 at 1/500 dilution (green). Right: Blue staining represents cells costained with Hoechst 33343.