Overview

  • Product name
    Anti-HIP55 antibody - N-terminal
    See all HIP55 primary antibodies
  • Description
    Rabbit polyclonal to HIP55 - N-terminal
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within N terminal amino acids 1- 44 of Human HIP55 (Uniprot ID: Q9UJU6).

  • Positive control
    • HeLa S3 and MOLT4 whole cell lysate; HeLa cells; Human H1299 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 89% PBS, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 48 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000. Fixation in ice cold methanol is recommended.

Target

  • Function
    Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin.
  • Sequence similarities
    Belongs to the ABP1 family.
    Contains 1 ADF-H domain.
    Contains 1 SH3 domain.
  • Domain
    The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1.
  • Post-translational
    modifications
    Degraded by caspases during apoptosis.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABP1 antibody
    • Actin-binding protein 1 antibody
    • Cervical mucin associated protein antibody
    • Cervical mucin-associated protein antibody
    • Cervical SH3P7 antibody
    • CMAP antibody
    • Dbnl antibody
    • DBNL_HUMAN antibody
    • Drebrin F antibody
    • drebrin like antibody
    • Drebrin like protein antibody
    • Drebrin-F antibody
    • Drebrin-like protein antibody
    • HIP 55 antibody
    • HIP-55 antibody
    • HPK1 interacting protein of 55 kDa antibody
    • HPK1-interacting protein of 55 kDa antibody
    • SH3 domain containing protein 7 antibody
    • SH3 domain-containing protein 7 antibody
    • SH3P7 antibody
    • src homology 3 domain containing protein HIP 55 antibody
    see all

Images

  • All lanes : Anti-HIP55 antibody - N-terminal (ab154016) at 1/1500 dilution

    Lane 1 : HeLa S3 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 48 kDa
  • Immunohistochemical analysis of paraffin-embedded Human H1299 xenograft tissue labeling HIP55 with ab154016 at 1/100 dilution.
  • Immunofluorescent analysis of HeLa cells (fixed in iced-cold Methanol for 5 min) labeling HIP55 with ab154016 at 1/500 dilution (green). Right: Blue staining represents cells costained with Hoechst 33343.

References

ab154016 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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