Overview

  • Product nameAnti-HIPK1 antibody
    See all HIPK1 primary antibodies
  • Description
    Mouse polyclonal to HIPK1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Macaque Monkey
  • Immunogen

    Recombinant fragment: VRQQQSSSLP SKKNKQSAPV SSKSSLDVLP SQVYSLVGSS PLRTTSSYNS LVPVQDQHQP IIIPDTPSPP VSVITIRSDT DEEEDNKYKP SSSGLKPRSN , corresponding to amino acids 833-932 of Human HIPK1

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab43641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 131 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • FunctionMay play a role as a corepressor for homeodomain transcription factors. Phosphorylates DAXX in response to stress, and mediates its translocation from the nucleus to the cytoplasm. May be involved in malignant squamous cell tumor formation.
  • Tissue specificityUbiquitously expressed with highest levels in skeletal muscle and heart. Overexpressed in breast cancer cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated. Phosphorylated and activated by JNK1.
  • Cellular localizationNucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hipk 1 antibody
    • Hipk1 antibody
    • HIPK1_HUMAN antibody
    • Homeodomain interacting protein kinase 1 antibody
    • Homeodomain interacting protein kinase 1 like protein antibody
    • Homeodomain-interacting protein kinase 1 antibody
    • Myak antibody
    • Nbak 2 antibody
    • Nbak2 antibody
    • Nuclear body associated kinase 2 antibody
    • Nuclear body associated kinase 2b antibody
    see all

Anti-HIPK1 antibody images

  • All lanes : Anti-HIPK1 antibody (ab43641) at 1/1000 dilution

    Lane 1 : ~20ug of a total protein extract from E coli containing 50ng of a tagged fusion protein of an irrelevant antigen
    Lane 2 : ~20ug of a total protein extract from E coli with 50ng of the antigen (tagged fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 5000

    Predicted band size : 131 kDa

References for Anti-HIPK1 antibody (ab43641)

ab43641 has not yet been referenced specifically in any publications.

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