Overview

  • Product name
    Anti-HIPK4 antibody
  • Description
    Rabbit polyclonal to HIPK4
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from an internal internal of human HIPK4.

  • Positive control
    • A549 clls; HUVEC cells; testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab69565 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

Images

  • All lanes : Anti-HIPK4 antibody (ab69565) at 1/500 dilution

    Lane 1 : extracts from HUVEC cells
    Lane 2 : extracts from HUVEC cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Immunohistochemistry analysis of paraffin-embedded human testis tissue using 1:50 ab69565. For the right hand image the antibody was pre-incubated with the immunizing peptide.
  • Immunofluorescence analysis of A549 cells, using 1:500 ab69565. For the right hand image, the antibody was pre-incubated with the immunizing peptide.

References

ab69565 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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