Overview

  • Product nameAnti-HIPK4 antibody
    See all HIPK4 primary antibodies
  • Description
    Mouse monoclonal to HIPK4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Recombinant fragment: RGSSWEEGEH LGASAEPLAI LQRDEDGPNI DNMTMEAERP DPELFDPSSC PGEWLSEPDC TLESVRGPRA QGLPPRRSHQ HGPPRGATSF LQHVTGHH, corresponding to amino acids 519-616 of Human HIPK4 (AAH34501) with a proprietory tag.

  • Positive control
    • HIPK4 transfected 293T cell lysate. Recombinant tagged human HIPK4 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 69 kDa.
ELISA Use at an assay dependent dilution.

Target

  • FunctionProtein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIPK 4 antibody
    • HIPK4 antibody
    • HIPK4_HUMAN antibody
    • Homeodomain interacting protein kinase 4 antibody
    • Homeodomain-interacting protein kinase 4 antibody
    see all

Anti-HIPK4 antibody images

  • Anti-HIPK4 antibody (ab77651) at 1 µg/ml + Recombinant tagged human HIPK4 fragment at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 69 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-HIPK4 antibody (ab77651) at 1 µg/ml

    Lane 1 : HIPK4 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 69 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)

References for Anti-HIPK4 antibody (ab77651)

ab77651 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"