The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 49 kDa).
FunctionRequired for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
Tissue specificityPresent in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
Sequence similaritiesBelongs to the IFT57 family.
DomainThe pseudo DED region (pDED) meadiates the interaction with HIP1.
Cellular localizationCytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.