Overview

  • Product nameAnti-HIPPI antibody
    See all HIPPI primary antibodies
  • Description
    Mouse monoclonal to HIPPI
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Opossum
  • Immunogen

    Recombinant fragment with tag: QTYHLDMNET AKQEDILEST TDAAEWSLEV ERVLPQLKVT IRTDNKDWRI HVDQMHQHRS GIESALKETK GFLDKLHNEI TRTLEKISSR EKYINNQLE, corresponding to amino acids 214-313 of Human HIPPI

  • Positive control
    • HIPPI transfected 293T cell lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab66241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 49 kDa).

Target

  • FunctionRequired for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
  • Tissue specificityPresent in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
  • Sequence similaritiesBelongs to the IFT57 family.
  • DomainThe pseudo DED region (pDED) meadiates the interaction with HIP1.
  • Cellular localizationCytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDNA FLJ10147 fis clone HEMBA1003369 antibody
    • Dermal papilla derived protein 8 antibody
    • Dermal papilla-derived protein 8 antibody
    • DERP 8 antibody
    • DERP8 antibody
    • Esrrbl 1 antibody
    • Esrrbl1 antibody
    • ESRRBL1 protein antibody
    • Estrogen related receptor beta like 1 antibody
    • Estrogen related receptor beta like protein 1 antibody
    • Estrogen-related receptor beta-like protein 1 antibody
    • FLJ10147 antibody
    • Hip1 Interacting protein antibody
    • HIP1 interacting protein HIPPI antibody
    • Hip1 protein interactor antibody
    • HIP1-interacting protein antibody
    • HIPPI antibody
    • Huntingtin interacting protein 1 interacting protein antibody
    • Huntingtin interacting protein 1 protein interactor antibody
    • IFT 57 antibody
    • ift57 antibody
    • IFT57_HUMAN antibody
    • Intraflagellar transport 57 homolog (Chlamydomonas) antibody
    • Intraflagellar transport 57 homolog antibody
    • Intraflagellar transport protein 57 homolog antibody
    • MHS4R2 antibody
    • Vestrogen related receptor beta like 1 antibody
    see all

Anti-HIPPI antibody images

  • All lanes : Anti-HIPPI antibody (ab66241) at 1 µg/ml

    Lane 1 : HIPPI transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 49 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 27 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HIPPI antibody (ab66241)

ab66241 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66241.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"