Anti-Hippocalcin antibody (ab24560)
Key features and details
- Rabbit polyclonal to Hippocalcin
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-Hippocalcin antibody
See all Hippocalcin primary antibodies -
Description
Rabbit polyclonal to Hippocalcin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish
Predicted to work with: Chicken, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: primary hippocampal rat neurons/glia, DIV14. cells WB: Rat Brain, Mouse Brain. IP: Mouse Brain.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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IP |
Use at an assay dependent concentration.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
IP
Use at an assay dependent concentration. |
Target
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Function
May be involved in the calcium-dependent regulation of rhodopsin phosphorylation. Binds two calcium ions. -
Tissue specificity
Brain specific. -
Sequence similarities
Belongs to the recoverin family.
Contains 4 EF-hand domains. -
Post-translational
modificationsMyristoylation facilitates interaction with membranes. - Information by UniProt
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Database links
- Entrez Gene: 509772 Cow
- Entrez Gene: 3208 Human
- Entrez Gene: 15444 Mouse
- Entrez Gene: 29177 Rat
- Omim: 142622 Human
- SwissProt: Q4PL64 Cow
- SwissProt: P84074 Human
- SwissProt: P84075 Mouse
see all -
Alternative names
- BDR 2 antibody
- BDR2 antibody
- Calcium binding protein BDR 2 antibody
see all
Images
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ab24560 staining Hippocalcin in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24560 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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All lanes : Anti-Hippocalcin antibody (ab24560) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 14 kDa (possible cleavage fragment), 42 kDa, 52 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Hippocalcin was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to Hippocalcin and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24560.
Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
Band: 23kDa: Hippocalcin; non specific - 60kDa: We are unsure as to the identity of this extra band. -
ICC/IF image of ab24560 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24560 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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All lanes : Anti-Hippocalcin antibody (ab24560) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate (20ug)
Lane 3 : Mouse brain homogenate (20ug)
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 1 minute
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab24560 has been referenced in 10 publications.
- Ratié L et al. Loss of Dmrt5 Affects the Formation of the Subplate and Early Corticogenesis. Cereb Cortex 30:3296-3312 (2020). PubMed: 31845734
- Lipinski M et al. KAT3-dependent acetylation of cell type-specific genes maintains neuronal identity in the adult mouse brain. Nat Commun 11:2588 (2020). PubMed: 32444594
- Norimoto H et al. A claustrum in reptiles and its role in slow-wave sleep. Nature 578:413-418 (2020). PubMed: 32051589
- Key J et al. Ubiquitylome profiling of Parkin-null brain reveals dysregulation of calcium homeostasis factors ATP1A2, Hippocalcin and GNA11, reflected by altered firing of noradrenergic neurons. Neurobiol Dis 127:114-130 (2019). PubMed: 30763678
- Ji J et al. Proteomic identification of hippocalcin and its protective role in heatstroke-induced hypothalamic injury in mice. J Cell Physiol 234:3775-3789 (2019). PubMed: 30256386
- Kang MJ et al. MicroRNA-24-3p regulates neuronal differentiation by controlling hippocalcin expression. Cell Mol Life Sci 76:4569-4580 (2019). PubMed: 31486848
- Koh PO Ferulic acid prevents cerebral ischemic injury-induced reduction of hippocalcin expression. Synapse 67:390-8 (2013). PubMed: 23401261
- Magnani D et al. Gli3 Controls Subplate Formation and Growth of Cortical Axons. Cereb Cortex : (2012). PubMed: 22903314
- Kathiresan T et al. A protein interaction network for the large conductance Ca(2+)-activated K(+) channel in the mouse cochlea. Mol Cell Proteomics 8:1972-87 (2009). PubMed: 19423573
- Osheroff H & Hatten ME Gene expression profiling of preplate neurons destined for the subplate: genes involved in transcription, axon extension, neurotransmitter regulation, steroid hormone signaling, and neuronal survival. Cereb Cortex 19 Suppl 1:i126-34 (2009). PubMed: 19398467