Overview

  • Product nameAnti-HIR1 antibody
    See all HIR1 primary antibodies
  • Description
    Rabbit polyclonal to HIR1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 800 to the C-terminus of Saccharomyces cerevisiae HIR1.

    (Peptide available as ab61633.)

  • Positive control
    • This antibody gave a positive signal in Saccharomyces cerevisiae Yeast Extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab46878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceHIR1 is a member of the HIR complex that also contains HIR2, HIR3 and HPC2. This complex is responsible for the repression of histone gene transcription as well as having a role in nucleosome assembly.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • Histone transcription regulator 1 antibody
      • YBL008W antibody

    Anti-HIR1 antibody images

    • Anti-HIR1 antibody (ab46878) at 1 µg/ml + S Cerevisiae Yeast Extract at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 94 kDa
      Observed band size : 94 kDa
      Additional bands at : 15 kDa,50 kDa. We are unsure as to the identity of these extra bands.
    • ICC/IF image of ab46878 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46878, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml.

    References for Anti-HIR1 antibody (ab46878)

    ab46878 has not yet been referenced specifically in any publications.

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