Overview

  • Product nameAnti-HIR1 antibody
    See all HIR1 primary antibodies
  • Description
    Rabbit polyclonal to HIR1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Saccharomyces cerevisiae HIR1.

    (Peptide available as ab61634.)

  • Positive control
    • This antibody gave a positive signal in Saccharomyces cerevisiae Yeast Extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab61635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 1 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHIR1 is a member of the HIR complex that also contains HIR2, HIR3 and HPC2. This complex is responsible for the repression of histone gene transcription as well as having a role in nucleosome assembly.
    • Cellular localizationNuclear
    • Database links
      • Alternative names
        • Histone transcription regulator 1 antibody
        • YBL008W antibody

      Anti-HIR1 antibody images

      • Anti-HIR1 antibody (ab61635) at 1 µg/ml + S cerevisiae Yeast Extract at 10 µg

        Secondary
        Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Performed under reducing conditions.

        Predicted band size : 94 kDa
        Observed band size : 94 kDa
        Additional bands at : 15 kDa,50 kDa. We are unsure as to the identity of these extra bands.
      • ICC/IF image of ab61635 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61635, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa, Hek293 and HepG2 cells at 1µg/ml, and in 4% formaldehyde fixed (10 min) HeLa cells at 1µg/ml.

      References for Anti-HIR1 antibody (ab61635)

      ab61635 has not yet been referenced specifically in any publications.

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"