The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHIR3 is a member of the HIR complex that also contains HIR1, HIR2 and HPC2. The HIR complex is responsible for the repression of histone gene transcription as well having a role in nucleosome assembly.