Overview

  • Product nameAnti-HIRA antibody
    See all HIRA primary antibodies
  • Description
    Rabbit polyclonal to HIRA
  • Specificityab20655 recognises a band of 89kDa corresponding to the short isoform of HIRA in HeLa, HeLa Nuclear, A431 and HEK293 lysates.
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human HIRA.

    (Peptide available as ab22456.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysate: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab20655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA (predicted molecular weight: 89 kDa). Can be blocked with HIRA peptide (ab22456).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
    • Tissue specificityExpressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
    • Sequence similaritiesBelongs to the WD repeat HIR1 family.
      Contains 8 WD repeats.
    • Developmental stageExpressed during embryogenesis.
    • Post-translational
      modifications
      Sumoylated.
      Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
    • Cellular localizationNucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
    • Information by UniProt
    • Database links
    • Alternative names
      • DGCR1 antibody
      • DiGeorge critical region gene 1 antibody
      • HIR antibody
      • HIR histone cell cycle regulation defective homolog A antibody
      • HIRA antibody
      • HIRA protein antibody
      • HIRA_HUMAN antibody
      • Histone cell cycle regulator antibody
      • Protein HIRA antibody
      • TUP 1 antibody
      • TUP1 like enhancer of split protein 1 antibody
      • TUP1-like enhancer of split protein 1 antibody
      • TUPLE1 antibody
      see all

    Anti-HIRA antibody images

    • Anti-HIRA antibody (ab20655) at 1 µg/ml + HepG2 nuclear extract lysate (ab14660) at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 89 kDa
      Observed band size : 89 kDa
      Additional bands at : 37 kDa (possible cross reactivity).

    References for Anti-HIRA antibody (ab20655)

    This product has been referenced in:
    • Majumder A  et al. Histone Chaperone HIRA in Regulation of Transcription Factor RUNX1. J Biol Chem 290:13053-63 (2015). Read more (PubMed: 25847244) »
    • Lewis PW  et al. Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres. Proc Natl Acad Sci U S A 107:14075-80 (2010). ChIP ; Mouse . Read more (PubMed: 20651253) »

    See all 3 Publications for this product

    Product Wall

    Abreviews
    Application Western blot
    Loading amount 10 cells
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris gel, precasted)
    Sample Zebrafish Tissue lysate - whole (10 whole embryo lysate, deyolked)
    Specification 10 whole embryo lysate, deyolked
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Maximilian Krause

    Verified customer

    Submitted Feb 03 2014

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More



    I have checked SwissProte, and it seems that there are two isoforms: one with 112kDa, and one with 89 kDa, which would match perfectly. You canfind the database link on ourdatasheet.

    In addition, I found that we received almost thee...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (Hela HEK293 NIH-3T3 MEF)
    Loading amount 20 µg
    Specification Hela HEK293 NIH-3T3 MEF
    Gel Running Conditions Reduced Denaturing (Precise protein gel 8-16%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Jun 16 2009

    Thank you for your enquiry. I have been in touch with my chromatin business development colleague and the size of the band that this antibody detects (89KDa) can be explained by the splice variant shown at the following SwissProt link: http://u...

    Read More

    Thank you for your enquiry. Ab20655 recognises a band at 89kDa which corresponds to the short isoform of HIRA. This band was specifically blocked by the immunising peptide. I hope this information helps, please do not hesitate to contact us if y...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa and Indian muntjac)
    Specification HeLa and Indian muntjac
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Apr 20 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"