Overview

  • Product name
  • Description
    Rabbit polyclonal to HIRA
  • Specificity
    ab20655 recognises a band of 89kDa corresponding to the short isoform of HIRA in HeLa, HeLa Nuclear, A431 and HEK293 lysates.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human HIRA.

    (Peptide available as ab22456.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysate: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab20655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).

Detects a band of approximately 89 kDa, corresponding to the short isoform of HIRA.

Target

  • Function
    Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • Tissue specificity
    Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
  • Sequence similarities
    Belongs to the WD repeat HIR1 family.
    Contains 8 WD repeats.
  • Developmental stage
    Expressed during embryogenesis.
  • Post-translational
    modifications
    Sumoylated.
    Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
  • Cellular localization
    Nucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGCR1 antibody
    • DiGeorge critical region gene 1 antibody
    • HIR antibody
    • HIR histone cell cycle regulation defective homolog A antibody
    • HIRA antibody
    • HIRA protein antibody
    • HIRA_HUMAN antibody
    • Histone cell cycle regulator antibody
    • Protein HIRA antibody
    • TUP 1 antibody
    • TUP1 like enhancer of split protein 1 antibody
    • TUP1-like enhancer of split protein 1 antibody
    • TUPLE1 antibody
    see all

Images

  • Anti-HIRA antibody (ab20655) at 1 µg/ml + HepG2 nuclear extract lysate (ab14660) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 37 kDa (possible cross reactivity).

    ab20655 recognises a band at 89kDa, corresponding to the short isoform of HIRA.

References

This product has been referenced in:

See all 5 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Western blot
Loading amount
10 cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel, precasted)
Sample
Zebrafish Tissue lysate - whole (10 whole embryo lysate, deyolked)
Specification
10 whole embryo lysate, deyolked
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Maximilian Krause

Verified customer

Submitted Feb 03 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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I have checked SwissProte, and it seems that there are two isoforms: one with 112kDa, and one with 89 kDa, which would match perfectly. You canfind the database link on ourdatasheet.

In addition, I found that we received almost thee...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela HEK293 NIH-3T3 MEF)
Loading amount
20 µg
Specification
Hela HEK293 NIH-3T3 MEF
Gel Running Conditions
Reduced Denaturing (Precise protein gel 8-16%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Jun 16 2009

Thank you for your enquiry. I have been in touch with my chromatin business development colleague and the size of the band that this antibody detects (89KDa) can be explained by the splice variant shown at the following SwissProt link: http://u...

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Thank you for your enquiry. Ab20655 recognises a band at 89kDa which corresponds to the short isoform of HIRA. This band was specifically blocked by the immunising peptide. I hope this information helps, please do not hesitate to contact us if y...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa and Indian muntjac)
Specification
HeLa and Indian muntjac
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 20 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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