Anti-HIRA (phospho T555) antibody (ab61095)

Overview

  • Product nameAnti-HIRA (phospho T555) antibody
    See all HIRA primary antibodies
  • Description
    Rabbit polyclonal to HIRA (phospho T555)
  • Specificityab61095 detects endogenous levels of HIRA only when phosphorylated at threonine 555.
  • Tested applicationsSuitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized phosphopeptide derived from human HIRA around the phosphorylation site of threonine 555 (L-T-TP-P-S).

  • Positive control
    • HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab61095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • FunctionCooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.
  • Tissue specificityExpressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.
  • Sequence similaritiesBelongs to the WD repeat HIR1 family.
    Contains 8 WD repeats.
  • Developmental stageExpressed during embryogenesis.
  • Post-translational
    modifications
    Sumoylated.
    Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.
  • Cellular localizationNucleus. Nucleus > PML body. Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence.
  • Information by UniProt
  • Database links
  • Alternative names
    • DGCR1 antibody
    • DiGeorge critical region gene 1 antibody
    • HIR antibody
    • HIR histone cell cycle regulation defective homolog A antibody
    • HIRA antibody
    • HIRA protein antibody
    • HIRA_HUMAN antibody
    • Histone cell cycle regulator antibody
    • Protein HIRA antibody
    • TUP 1 antibody
    • TUP1 like enhancer of split protein 1 antibody
    • TUP1-like enhancer of split protein 1 antibody
    • TUPLE1 antibody
    see all

Anti-HIRA (phospho T555) antibody images

  • Immunofluorescence analysis of HeLa cells, using ab61095 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

References for Anti-HIRA (phospho T555) antibody (ab61095)

ab61095 has not yet been referenced specifically in any publications.

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