Anti-HIRIP3 antibody (ab99208)

Overview

  • Product name
  • Description
    Rabbit polyclonal to HIRIP3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 395-444 (RTRSSSSSSD GSPEAKGGKA GSGRRGEDHP AVMRLKRYIR ACGAHRNYKK) of Human HIRIP3 (NP_003600).

  • Positive control
    • ACHN cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.
  • Tissue specificity
    Widely expressed. Isoform 1 is predominant in skeletal muscle. Isoform 2 is predominant in liver and heart.
  • Post-translational
    modifications
    Phosphorylated by CK2.
  • Cellular localization
    Nucleus. Nuclear throughout the cell cycle and is excluded from condensed chromatin during mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • HIRA-interacting protein 3 antibody
    • HIRIP3 antibody
    • HIRP3 antibody
    • HIRP3_HUMAN antibody

Images

  • Anti-HIRIP3 antibody (ab99208) at 1 µg/ml + ACHN cell lysate at 10 µg

    Predicted band size : 62 kDa

References

ab99208 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (different cell lines)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
different cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF and whole brain lysate)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
40 µg
Specification
MEF and whole brain lysate
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2016

Thank you for your answer.

I do completely understand your concerns. I am happy to see that the ab99208 does seem to provide finally good results. I am sorry to hear that the results with ab100856 are unsatisfactory.

Indeed, both an...

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Thank you for taking time to complete our questionnaire. I am sorry that this antibody is not providing satisfactory results.

Having reviewed this case, I would like to offer some suggestions to help optimize the results from ab99208. I woul...

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I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

We a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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