Anti-Hirudin antibody [2D7] (ab17198)

Overview

  • Product name
    Anti-Hirudin antibody [2D7]
  • Description
    Mouse monoclonal [2D7] to Hirudin
  • Specificity
    The affinity (Kd) of this antibody for rHV2K47 is 4.5x10E-7 M
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Epitope
    The epitope of this antibody is located at the C-terminal end of hirudin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    2D7
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/2000.
    WB: Use at an assay dependent dilution. ab17198 reacts with hirudin in both reduced and unreduced forms. Detects a band of approximately 7 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Hirudin is a highly effective and very specific anticoagulant which specifically inhibits formation of fibrin and activation of factors V, VIII and XIII by forming irreversible, noncovalent 1:1 complexes with a-thrombin. Hirudin is a 65 amino acid polypeptide, 7 kDa protein naturally originating from the medical leech Hirudo medicinalis. It is the most potent thrombin inhibitor known to date.
    • Cellular localization
      Secreted
    • Alternative names
      • Hirudin 1 antibody
      • Lepirudin antibody
      • Refludan antibody

    References

    ab17198 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for contacting Abcam.
    I have heard back from the lab and they have told me that ab17198reacts to the variant 2 form of Hirudin, not variant 1.
    I am sorry that I could not help you further in this matter, but if there is anything els...

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    Thank you for contacting us.

    We test ab17198 and ab36390 byaccessing their binding abilities to human thrombin and to complex of human thrombin with the thromobin inhibitor hirudin. We have not tested the antibodies in other species other th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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