Overview

  • Product name
    His-tagged Protein Gold Detection Kit
  • Product overview

    His-tagged Protein Gold Detection Kit (ab170734) is intended and optimized for direct visualization of recombinant His-tagged proteins and other proteins bearing different histidine tags in western or dot blotting applications. Unlike detection by anti-6xHis antibodies, His-tagged Protein Gold Detection does not require a specific location of the polyhistidine tag (N- or C-terminus), does not need primary and secondary antibody incubations, or the presence of specific adjacent amino acid sequences.

    His-tagged Protein Gold Detection Kit uses Nickel-NTA (nickel-nitrilotriacetic acid)-functionalized gold nanoparticles to specifically bind to His-tagged proteins. With the autometallographic amplification subsequently applied to the gold nanoparticles, His-tagged Protein Gold Detection allows the direct visualization of His-tagged proteins. His-tagged Protein Gold Detection generates specific purple colored metallic bands or dots which do not fade and will not dissolve in water and organic solvents. The His-tagged Protein Gold Detection Kit can detect nanogram levels of purified His-tagged proteins. The entire procedure takes about 1 hour.

  • Tested applications
    Suitable for: WB, Dot blotmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab170734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.

Protocols

References

ab170734 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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