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His-tagged Protein Gold Detection Kit (ab170734) is intended and optimized for direct visualization of recombinant His-tagged proteins and other proteins bearing different histidine tags in western or dot blotting applications. Unlike detection by anti-6xHis antibodies, His-tagged Protein Gold Detection does not require a specific location of the polyhistidine tag (N- or C-terminus), does not need primary and secondary antibody incubations, or the presence of specific adjacent amino acid sequences.
His-tagged Protein Gold Detection Kit uses Nickel-NTA (nickel-nitrilotriacetic acid)-functionalized gold nanoparticles to specifically bind to His-tagged proteins. With the autometallographic amplification subsequently applied to the gold nanoparticles, His-tagged Protein Gold Detection allows the direct visualization of His-tagged proteins. His-tagged Protein Gold Detection generates specific purple colored metallic bands or dots which do not fade and will not dissolve in water and organic solvents. The His-tagged Protein Gold Detection Kit can detect nanogram levels of purified His-tagged proteins. The entire procedure takes about 1 hour.
|AutoMet Detect A||1 x 40ml|
|AutoMet Detect B||1 x 40ml|
|AutoMet Detect C||1 x 40ml|
|AutoMet Detect D||1 x 40ml|
|Nickel-NTA-Gold||1 x 1.5ml|
Our Abpromise guarantee covers the use of ab170734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
ab170734 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"