Overview

  • Product name
    Anti-HIST1H2AK antibody
  • Description
    Rabbit polyclonal to HIST1H2AK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Monkey, Zebrafish
  • Immunogen

    Synthetic peptide within Human HIST1H2AK aa 63-87 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    ILELAGNA ARDNKKTRII PRHLQLA


    Database link: P0C0S8

  • Positive control
    • HeLa, HepG2 and HL60 cell lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab191237 was purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 14 kDa.

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H2A family.
  • Post-translational
    modifications
    The chromatin-associated form is phosphorylated on Thr-121 during mitosis.
    Deiminated on Arg-4 in granulocytes upon calcium entry.
    Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosome chromatin. Ubiquitination of H2A functions downstream of methylation of 'Lys-27' of histone H3. Monoubiquitination of Lys-120 by RNF2/RING2 can also be induced by ultraviolet and may be involved in DNA repair. Following DNA double-strand breaks (DSBs), it is ubiquitinated through 'Lys-63' linkage of ubiquitin moieties by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168, leading to the recruitment of repair proteins to sites of DNA damage. Monoubiquitination and ionizing radiation-induced 'Lys-63'-linked ubiquitination are distinct events.
    Phosphorylation on Ser-2 is enhanced during mitosis. Phosphorylation on Ser-2 by RPS6KA5/MSK1 directly represses transcription. Acetylation of H3 inhibits Ser-2 phosphorylation by RPS6KA5/MSK1.
    Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H2A histone family, member P antibody
    • H2A.1 antibody
    • H2A.1b antibody
    • H2A/n antibody
    • H2A/p antibody
    • H2A1_HUMAN antibody
    • H2AF antibody
    • H2AFC antibody
    • H2AFD ANDHIST1H2AL antibody
    • H2AFD antibody
    • H2AFI antibody
    • H2AFN ANDHIST1H2AG antibody
    • H2AFN antibody
    • H2AFP antibody
    • HIST1H2AI antibody
    • HIST1H2AJ antibody
    • HIST1H2AK antibody
    • HIST1H2AL antibody
    • HIST1H2AM antibody
    • Histone 1, H2ag antibody
    • Histone cluster 1, H2ag antibody
    • Histone H2A type 1 antibody
    • Histone H2A.c/d/i/n/p antibody
    • Histone H2A/p antibody
    • pH2A/f antibody
    see all

Images

  • All lanes : Anti-HIST1H2AK antibody (ab191237) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HL60 cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 14 kDa

References

ab191237 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191237.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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