Overview

  • Product name
    Anti-HIST1H2BO antibody
  • Description
    Rabbit polyclonal to HIST1H2BO
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HIST1H2BO aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P23527

  • Positive control
    • NCI-H460 cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab113033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 14 kDa.

Target

  • Function
    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • Sequence similarities
    Belongs to the histone H2B family.
  • Post-translational
    modifications
    Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II.
    Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class-switch recombination.
  • Cellular localization
    Nucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • H2B/n antibody
    • H2B1O_HUMAN antibody
    • H2BFH antibody
    • H2BFN antibody
    • HIST1H2BO antibody
    • Histone H2B type 1-O antibody
    • Histone H2B.2 antibody
    • Histone H2B.n antibody
    see all

Images

  • Anti-HIST1H2BO antibody (ab113033) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 14 kDa

References

ab113033 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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