Overview

  • Product nameAnti-Histamine antibody
    See all Histamine primary antibodies
  • Description
    Rabbit polyclonal to Histamine
  • SpecificityUsing a conjugate Histamine-Glutaraldehyde-Protein, antibody specifity was performed with an ELISA test by competition experiments with the following compounds:
    Compound Cross-reactivity ratio*
    Histamine-G-BSA 1
    1-Methylhistamine-G-BSA 1/>50,000
    3-Methylhistamine-G-BSA 1/>50,000
    Histidine-G-BSA 1/>50,000
    *Histamine-G-BSA concentration/conjugated amino acid concentration at half displacement
  • Tested applicationsSuitable for: ELISAmore details
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

Properties

Applications

Our Abpromise guarantee covers the use of ab6449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/5000.

Target

  • RelevanceHistamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. Histamine causes running nose, sneezing and itching, and narrows the airways in the lungs. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon.
  • Cellular localizationSecreted
  • Alternative names
    • 2 (4 imidazolyl)ethylamine antibody
    • 4 (2 aminoethyl) 1 3 diazole antibody

References for Anti-Histamine antibody (ab6449)

ab6449 has not yet been referenced specifically in any publications.

Product Wall

Ab6449 is anti-histamine polyclonal antibodies. They are produced on rabbits but you can detect histamine in human tissue.

We have no information on cross-reactivity of this antibody with serum proteins, but ELISA tests with this antibody are ongoing and we will update the datasheet as soon as new data are available.

This antibody has not been tested for blocking ability. Applications in which it has been tested are ELISAs and IHC (see cross-reactivity and references sections of the datasheet). We do not have a Canadian supplier; you can order direct from Abcam i...

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It has not been tested for cross-reactivity against polyamines; only against single residues of histamine, methyl histamine and histidine conjugated to BSA by glutaraldehyde (see cross-reactivity table on ddattasheet).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"