Overview

  • Product name
  • Description
    Rabbit polyclonal to Histidase
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 180-229 (

    INKLQELQVNLVRSHSSGVGKPLSPERCRMLLALRINVLAKGYSGISLET

    ) of Human Histidase (NP_002099)

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab83831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 1.25 µg/ml.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human fetal liver tissue labelling Histidase with ab83831 at 1.25µg/ml.

  • Anti-Histidase antibody (ab83831) at 1 µg/ml (in 5% skim milk / PBS buffer) + 10ug 721_B cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000 - 1/100,000

    Predicted band size : 73 kDa
    Observed band size : 73 kDa
    Gel concentration 12%

References

ab83831 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab83831.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up