• Product nameAnti-Histone Core antibody
  • Description
    Sheep polyclonal to Histone Core
  • SpecificityThe antibody binds to H1, H2B, H3 and H4. There is no evidence to suggest that the antibody can bind to H2A.
  • Tested applicationsSuitable for: IP, WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Human histone.

  • Positive control
    • Tonsil, colon carcinoma and any other human tissues.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPhosphate buffered saline
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab7832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: 1/1000.
    ICC/IF: Use at an assay dependent dilution (PMID 18478156).
    IP: Use at an assay dependent dilution.
    WB: 1/200.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceIn diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers. The histone core protein is an octamer consisting of 2 subunits of each of histone 2A, 2B, 3 and 4. In conjunction with the linker histone H1 and the nonhistone chromosomal proteins, it is responsible for the binding and compacting of DNA into chromatin. The sequences of the core histones are highly conserved from species to species.
    • Cellular localizationNuclear

    Anti-Histone Core antibody images

    References for Anti-Histone Core antibody (ab7832)

    This product has been referenced in:
    • Li Y  et al. Characterization of nucleohistone and nucleoprotamine components in the mature human sperm nucleus. Asian J Androl 10:535-41 (2008). ICC/IF ; Human . Read more (PubMed: 18478156) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HL60)
    Loading amount 10 µg
    Specification HL60
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Jul 13 2007

    The antibody (ab7832 -- Sheep polyclonal to Histone Core)binds to H1, H2B, H3 and H4; however, there is no evidence to suggest that the antibody can bind to H2A.

    This antibody has not been tested specifically in Drosophila, but it works in both animal and plant tissue, so I would expect it to work in Drosophila. Core histones are highly conserved.

    This antibody has not been tested in budding yeast, however as histones are highly conserved across species, it is quite likely that the antibody will react with yeast histones. As the antibody is raised against a mixture of histones, I think it likely...

    Read More