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Methylation of histone H3 at lysine 4 (H3K4) is thought to be a global epigenetic mark in euchromatin and a mediator of activated transcription. H3K4 demethylation is regulated by KDM1/LSD1, which can remove di- and mono-methylation from H3K4, and the JARID proteins such as RBP2 which catalyzes the removal of methylation through a hydroxylation reaction.
Abcam's Histone Demethylase (H3K4) Activity Quantification Assay Kit (ab113455) allows the user to measure activity/inhibition of H3K4 specific histone demethylases using cell/ tissue extracts. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase H3K4 specific activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 15ml||1 x 30ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Detection Antibody, 200 µg/mL||1 x 8µl||1 x 16µl|
|Fluoro Developer||1 x 12µl||1 x 24µl|
|Fluoro Dilution||1 x 4ml||1 x 8ml|
|Fluoro Enhancer||1 x 12µl||1 x 24µl|
|HDM Assay Buffer||1 x 1.5ml||1 x 3ml|
|HDM Standard, 20 µg/mL||1 x 25µl||1 x 50µl|
|HDM Substrate||1 x 50µl||1 x 100µl|
Our Abpromise guarantee covers the use of ab113455 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113455 has not yet been referenced specifically in any publications.