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KMD1/LSD1 (Lysine Specific Histone Demethylase 1) is a histone demethylase that only acts on mono- or di-methylated lysines. KDM1/LSD1 acts as a co-repressor by mediating demethylation of H3(meK4), a specific tag for epigenetic transcriptional activation.
Histone Demethylase KDM1/LSD1 Activity Quantification Assay Kit (ab113457) allows the user to measure activity/inhibition of KDM1/LSD1. The kit is ready-to-use and provides all the essential components needed for measuring histone demethylase KDM1/LSD1 activity/inhibition from a broad range of species including mammalian cells/tissues, plants, and bacteria.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 15ml||1 x 30ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Capture Antibody, 1000 µg/mL||1 x 5µl||1 x 10µl|
|Detection Antibody, 200 µg/mL||1 x 8µl||1 x 16µl|
|Fluoro-Developer||1 x 6ml||1 x 12µl|
|LSD1 Assay Buffer||1 x 2ml||1 x 4ml|
|LSD1 Assay Standard, 20 µg/mL||1 x 25µl||1 x 50µl|
|LSD1 Substrate||1 x 50µl||1 x 100µl|
Our Abpromise guarantee covers the use of ab113457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab113457 has not yet been referenced specifically in any publications.