Overview

  • Product nameHistone Extraction Kit
  • Tests
    1 x 100 test
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    ab113476 is suitable for a quick preparation of total histone extracts from mammalian cells and tissue samples. The minimal amount of starting materials can be as low as 105 cells or 1 mg of tissue. For the best results, the cell number should be greater than 106 cells or the tissue amount should be greater than 10 mg. A total of 100 standard extractions (use 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.

  • Notes

     

     

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab113476 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration. Total histone protein yield = 0.4mg/107 or 100mg tissue (yield may vary depending on cell or tissue type).

    Histone Extraction Kit images

    • Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.

      Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE

    • Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.

    Protocols

    References for Histone Extraction Kit (ab113476)

    ab113476 has not yet been referenced specifically in any publications.

    Product Wall

    The equivalent G Force values are:

    1000 RPM = 110G
    3000 RPM = 1000G
    10,000 RPM = 8000G
    12,000 RPM = 11,000G

    No special preparation such as desalting needs to be done before loading the sample into the gel.

    Abreviews
    The EpiSeeker Histone Extraction kit was used to isolate histone proteins for use with the EpiSeeker Histone H3 Total Acetylation Detection Fast Kit. Histones were extracted pupae of the flesh fly, Sarcophaga bullata, which is a non-model insect.

    The kit was easy and fast to use. It was easy to scale the reaction size to the amount of tissue.
    Username

    Dr. Julie Reynolds

    Verified customer

    Submitted Aug 21 2014

    Histone Extraction from a non-model insect

    Good Excellent 5/5 (Ease of Use)
    Abreviews
    This kit was used to isolate histones from pupae of the fleshy fly, Sarcophaga bullata, a non-model insect. A single pupa, weighing approximately 100 mg, was used in each sample. Each extraction yielded 14-19 ug/ul of histone protein.
    The kit was easy to use and simple to scale to the size of my insects. The extracted histones worked well with the EipSeeker Histone H3 Total Acetylation Detection Fast Kit.
    Username

    Abcam user community

    Verified customer

    Submitted Aug 20 2014

    ab113476 does not contain any HDAC inhibitors.

    The equivalent G force values are: 1000 RPM = 110G 3000 RPM = 1000G 10,000 RPM = 8000G 12,000 RPM = 11,000G

    I can confirm that ab113476: EpiSeeker Histone Extraction Kit would be suitable to use for human blood and skin and would extract total histone proteins, including H1, that would be quantifiable.

    Nuclear Protein extraction and Histone protein extraction from cell samples frozen at -80oC could still work using ab113474 and ab113476. However, the enzyme activity of nuclear extracts from frozen tissues may be much lower than that from fresh tissues.

    Ab113476 can be used to extract histones from a protozoan parasite if you use the protocol for histone extraction from tissues.

    yes ab113476 can be used for extracting histone proteins from frozen whole blood cells. For the best results, the amount of leukocyte cells in the whole blood should be estimated so that proper volume of whole blood can be used for extracting sufficien...

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    Hello! The two kits ab115102 and ab113476 should be suitable for use in fish, while ab133099 is designed to study inhibitors and so fish is not suitable. Please let me know if you have further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"