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Tissue, cells or virus corresponding to Human Histone H1. Immunogen is nuclei of myeloid leukemia biopsy cells.
Our Abpromise guarantee covers the use of ab62884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5µg for 106 cells.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 23 kDa).|
ab62884 staining Histone H1 in mouse primary hepatocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.1% Triton X-100 for 1 hour at 22°C. Samples were incubated with primary antibody (1/30 in blocking buffer) for 20 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.
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