Overview

  • Product nameAnti-Histone H1 antibody [0.T.60]See all Histone H1 primary antibodies ...
  • Description
    Mouse monoclonal [0.T.60] to Histone H1
  • SpecificityRecognizes and is specific for human histone H1 at ~32-33kD. Doublet detected due to hyper-phosphorylation of histone H1 subpopulation. Crossreacts with other histone isoforms in the following order of signal strength: H3, H2b > H4 > H2a, as determined with recombinant protein.
  • Tested applicationsICC, WB more details
  • Species reactivity
    Reacts with: Cow, Human, Plants
  • Immunogen

    Nuclei from myeloid leukemia biopsy cells.

  • General notesMolecular Weights of Histones: Lysine Rich (H1, f1): 21,500 Slightly Lysine Rich (H2a, f2a2): 14,004 Slightly Lysine Rich (H2b, f2b): 13,774 Arginine Rich (H3, f3): 15,324 Arginine Rich (H4, f2a1): 11,282

Properties

Applications

Our Abpromise guarantee covers the use of ab4269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use a concentration of 5 - 20 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 32-33 kDa.

Target

  • FunctionMay play a key role in the control of gene expression during oogenesis and early embryogenesis, presumably through the perturbation of chromatin structure. Essential for meiotic maturation of germinal vesicle-stage oocytes. The somatic type linker histone H1c is rapidly replaced by H1oo in a donor nucleus transplanted into an oocyte. The greater mobility of H1oo as compared to H1c may contribute to this rapid replacement and increased instability of the embryonic chromatin structure. The rapid replacement of H1c with H1oo may play an important role in nuclear remodeling.
  • Tissue specificityOocyte-specific.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Cellular localizationCytoplasm. Nucleus. Chromosome.
  • Target information above from: UniProt accession Q8IZA3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • dJ221C16.2 antibody
    • dJ221C16.5 antibody
    • DmeH1 antibody
    • H1 0 histone antibody
    • H1 antibody
    • H1 histone family member 0 antibody
    • H1 histone family member 1 antibody
    • H1 histone family member 3 antibody
    • H1 histone family member 5 antibody
    • H1 histone family member N testis specific antibody
    • H1 histone family member O oocyte specific antibody
    • H1 histone family member X antibody
    • H1(0) antibody
    • H1.1 antibody
    • H1.2 antibody
    • H1.3 antibody
    • H1.4 antibody
    • H1.5 antibody
    • H10 antibody
    • H1A antibody
    • H1f0 antibody
    • H1F0 protein antibody
    • H1F1 antibody
    • H1F2 antibody
    • H1F3 antibody
    • H1F4 antibody
    • H1F5 antibody
    • H1FNT antibody
    • H1FOO antibody
    • H1FOO protein antibody
    • H1FOO_HUMAN antibody
    • H1FT antibody
    • H1FV antibody
    • H1FX antibody
    • H1oo antibody
    • H1t antibody
    • H1T2 antibody
    • H1X antibody
    • HANP1 antibody
    • His1 antibody
    • HisC antibody
    • HIST1 antibody
    • HIST1H1A antibody
    • HIST1H1B antibody
    • HIST1H1C antibody
    • HIST1H1D antibody
    • HIST1H1E antibody
    • HIST1H1T antibody
    • Histone 1 H1a antibody
    • Histone 1 H1b antibody
    • Histone 1 H1c antibody
    • Histone 1 H1d antibody
    • Histone 1 H1e antibody
    • Histone 1 H1t antibody
    • Histone antibody
    • Histone H1' antibody
    • Histone H1.0 antibody
    • Histone H1.1 antibody
    • Histone H1.2 antibody
    • Histone H1.3 antibody
    • Histone H1.4 antibody
    • Histone H1.5 antibody
    • Histone H1oo antibody
    • Histone H1x antibody
    • MGC116819 antibody
    • MGC126630 antibody
    • MGC126632 antibody
    • MGC126642 antibody
    • MGC138176 antibody
    • MGC138345 antibody
    • MGC15959 antibody
    • MGC3992 antibody
    • MGC50807 antibody
    • MGC5241 antibody
    • MGC8350 antibody
    • Oocyte specific histone H1 antibody
    • Oocyte specific linker histone H1 antibody
    • Oocyte-specific histone H1 antibody
    • Oocyte-specific linker histone H1 antibody
    • osH1 antibody
    • Testicular H1 histone antibody
    see all

Anti-Histone H1 antibody [0.T.60] images



  • Predicted band size : 32-33 kDa


    Mouse monoclonal to Histone H1 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure.
    Dilution 1:1,000.
    Recombinant H1 isoforms courtesy of Dr Nicole Happel.

    Dr Albert Jordan, Center for Genomic Regulation (CRG).

References for Anti-Histone H1 antibody [0.T.60] (ab4269)

This product has been referenced in:
  • Stuss DP  et al. Impaired in vivo binding of MeCP2 to chromatin in the absence of its DNA methyl-binding domain. Nucleic Acids Res 41:4888-900 (2013). WB ; Mouse . Read more (PubMed: 23558747) »
  • Liu H  et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB ; Human . Read more (PubMed: 20818375) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

H1.2 specific antibodies,
ab4086; http://www.abcam.com/Histone-H1-2-antibody-ChIP-Grade-ab4086.html. (we however haven't tested the specificity to other forms)
ab17677; http://www.abcam.com/Histone-H1-2-...

Read More

Thank you for contacting us.

ab87556 was used with a sequence from H1FOO_HUMAN protein; http://www.uniprot.org/uniprot/Q8IZA3. According to swissprot the molecular weight is 35 kDa.

ab83058; the sequence used is from P07305 http://...

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Thank you for your interest.
Generally, we do not test our antibodies in insect species - mainly in mammalian system (human, mouse and rat).
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Unfortunately I am unable to find out what plants have been tested with ab4269, I'm sorry I couldn't help more. Please be assured that our guarantee of a credit note/refund will be offered if you decide to purchase this antibody and test it in Arab...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"