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Tissue, cells or virus corresponding to Human Histone H1. Immunogen is nuclei of myeloid leukemia biopsy cells.
Our Abpromise guarantee covers the use of ab62884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IF||Use a concentration of 2 µg/ml.
For better results, cells should be fixed with methanol.
|Flow Cyt||Use 0.5µg for 106 cells.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||1/100 - 1/500. Detects a band of approximately 17 kDa (predicted molecular weight: 23 kDa).|
ab62884 staining Histone H1 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab62884 at 2µg/ml and ab6046 (anti beta Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.
Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
ab62884 staining Histone H1 in mouse primary hepatocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 3% BSA + 0.1% Triton X-100 for 1 hour at 22°C. Samples were incubated with primary antibody (1/30 in blocking buffer) for 20 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/5000) was used as the secondary antibody.
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