Anti-Histone H1 antibody (ab61177)

Overview

  • Product nameAnti-Histone H1 antibody
    See all Histone H1 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1
  • SpecificityDetects total Histone H1 levels.
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (E-K-TP-P-V).

  • Positive control
    • IHC-P: Human normal colon FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab61177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 36 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

Anti-Histone H1 antibody images

  • All lanes : Anti-Histone H1 antibody (ab61177) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with synthesized peptide


    Predicted band size : 36 kDa
  • IHC image of ab61177 staining Histone H1 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61177, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab61177 at 1/500 - 1/1000 dilution staining Histone H1 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence.

References for Anti-Histone H1 antibody (ab61177)

ab61177 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10000 cells
Gel Running Conditions Reduced Denaturing (4-12% gradient)
Sample Human Cell lysate - nuclear (huh7 liver)
Specification huh7 liver
Treatment infected with flavivirus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 12 2014

Thank you for your enquiry.

Based on the immunogen sequence, ab61177 should recognize
Histone H1.5 (http://www.uniprot.org/uniprot/P16401),
Histone H1.3 (http://www.uniprot.org/uniprot/P16402), and
Histone H1.4 (http://www.uni...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"