Anti-Histone H1 (phospho ) antibody (ab4270)

Overview

  • Product name
    Anti-Histone H1 (phospho ) antibody
    See all Histone H1 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1 (phospho )
  • Specificity
    Recognizes phosphorylated histone H1
  • Tested applications
    Suitable for: Flow Cyt, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Hyper-phosphorylated isoform of tetrahymena Histone H1

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.035% Sodium Azide
    Constituents: 30% Glycerol, 0.7M Tris glycine, 0.115M Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 32 kDa. Additional proteins at 66 kD and 100 kD were detected. It detected Histone H1 from acid extracts of colcemid treated HeLa cells but not untreated HeLa cells. Acid extracts from colcemid treated and untreated HeLa cell were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-phospho- Histone H1 (2 µg/ml).
ICC Use a concentration of 5 - 10 µg/ml.

Target

Anti-Histone H1 (phospho ) antibody images

  • Rabbit polyclonal to Histone H1 (phospho) (1/100).

    Human neuroblastoma cell line SK-N-SH cultured on coverslips were fixed in 4% paraformaldehye and then stained with ab4270 (green). The DNA stained with DAPI is shown in red. (100x magnification).

  • All lanes : Anti-Histone H1 (phospho ) antibody (ab4270) at 2 µg/ml

    Lane 1 : Colcemid treated HeLa cell lysate
    Lane 2 : Untreated HeLa cell lysate

    Secondary
    Lane 1 : Goat anti-rabbit secondary antibody labeled with HRP
    Lane 2 : Goat anti-rabbit secondary antibody labeled with HRP.

    Developed using the ECL technique

References for Anti-Histone H1 (phospho ) antibody (ab4270)

This product has been referenced in:
  • Büchner A  et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). Read more (PubMed: 25391294) »
  • Chien WW  et al. S-phase lengthening induced by p16(INK4a) overexpression in malignant cells with wild-type pRb and p53. Cell Cycle 9: (2010). WB ; Human . Read more (PubMed: 20703084) »

See all 4 Publications for this product

Product Wall



Concerningab4270, Histone H1, phosphorylated PabRabbit x:



Thisantibody is produced by using hyper-phosphorylated histone H1 as the immunogen. The number and location of phosphorylation sites that thisantibody recognizes h...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Parotid gland)
Loading amount
100 µg
Specification
Parotid gland
Treatment
5Gy gamma irradiation
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1500µg/mL · Temperature: 20°C
Username

Dr. Kirsten Limesand

Verified customer

Submitted Nov 16 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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