This fast track antibody is not yet fully characterized. It is subject to these terms and conditions

Anti-Histone H1 (phospho S1 + T3) antibody (ab32979)

Overview

Properties

Applications

  • Application notesThis antibody gave a positive result in ELISA against the immunizing peptide (ab36684). It gave a negative result in ELISA against the non-modified equivalent peptide (ab36686). This indicates that it is specific for the modified peptide.
    See figure below.
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Histone H1 (phospho S1 + T3) antibody images

    This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.

    • All lanes : Anti-Histone H1 (phospho S1 + T3) antibody (ab32979) at 1 µg/ml

      Lane 1 : Calf Thymus Histone Preparation at 0.5 µg
      Lane 2 : Calf Thymus Histone Preparation at 0.5 µg with Human Histone H1 (phospho S1 + T3) peptide (ab36684) at 1 µg/ml
      Lane 3 : Calf Thymus Histone Preparation at 0.5 µg with Human Histone H1 peptide (ab36686) at 1 µg/ml
      Lane 4 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg
      Lane 5 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg with Human Histone H1 (phospho S1 + T3) peptide (ab36684) at 1 µg/ml
      Lane 6 : HeLa Histone Preparation Nuclear Lysate - Colcemid-treated at 2.5 µg with Human Histone H1 peptide (ab36686) at 1 µg/ml

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 32-36 kDa
    • This antibody gave a positive result in ELISA against the immunizing peptide ab36684 (blue line). It gave a negative result in ELISA against the non-modified equivalent peptide ab36686 (purple line). This indicates that it is specific for the modified peptide.    

    References for Anti-Histone H1 (phospho S1 + T3) antibody (ab32979)

    ab32979 has not yet been referenced specifically in any publications.

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