Purification notesab61060 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunofluorescence analysis, using Histone H1 (phospho T17) antibody (ab61060) at 1/500 - 1/1000 dilution, of HuvEc cells with serum treatment (20%, 30mins)(left panel) or without serum treatment (right panel).
References for Anti-Histone H1 (phospho T17) antibody (ab61060)
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