Anti-Histone H1 (phospho T17) antibody (ab61060)

Overview

  • Product nameAnti-Histone H1 (phospho T17) antibody
    See all Histone H1 primary antibodies
  • Description
    Rabbit polyclonal to Histone H1 (phospho T17)
  • Specificityab61060 detects endogenous levels of Histone H1 only when phosphorylated at threonine 17.
  • Tested applicationsSuitable for: ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Histone H1 around the phosphorylation site of threonine 17 (EKTpPV).

  • Positive control
    • HuvEc cells, treated with serum (20%, 30mins).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab61060 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab61060 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

Anti-Histone H1 (phospho T17) antibody images

  • Immunofluorescence analysis, using Histone H1 (phospho T17) antibody (ab61060) at 1/500 - 1/1000 dilution, of HuvEc cells with serum treatment (20%, 30mins)(left panel) or without serum treatment (right panel).

References for Anti-Histone H1 (phospho T17) antibody (ab61060)

ab61060 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"