Overview

  • Product nameAnti-Histone H1.0 antibody [34]
    See all Histone H1.0 primary antibodies
  • Description
    Mouse monoclonal [34] to Histone H1.0
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, ICC, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Xenopus laevis, all other VertebratesDoes not react with: Bird
  • Immunogen

    Ox Liver Histone H1.0.

  • EpitopeThis antibody recognises an epitope within aa20-30. Proline 26 is not essential for recognition by the antibody. See Gorka et al. 1998 for more information.
  • Positive control
    • Murine erythroleukemia (MEL) cells were used in part of the original characterisation of this antibody. Purified histones can also be used as a positive control. This antibody also gave a positive signal in human breast adenocarcinoma tissue sections. In Flow Cytometry, this antibody gave a positive signal in HeLa cells. ICC/IF - HeLa cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.4
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 0.4M arginine
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number34
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 20 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF Use a concentration of 10 µg/ml.

For better results, cells should be fixed with methanol.

ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
EMSA Use at an assay dependent concentration.

Target

  • FunctionHistones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.
  • Sequence similaritiesBelongs to the histone H1/H5 family.
    Contains 1 H15 (linker histone H1/H5 globular) domain.
  • Post-translational
    modifications
    Phosphorylated on Ser-17 in RNA edited version.
  • Cellular localizationNucleus. Chromosome. The RNA edited version has been localized to nuclear speckles. During mitosis, it appears in the vicinity of condensed chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • H1 histone family member 0 antibody
    • H1(0) antibody
    • H10 antibody
    • H10_HUMAN antibody
    • h1f0 antibody
    • H1FV antibody
    • Histone H1'' antibody
    • Histone H1(0) antibody
    • Histone H1.0 antibody
    • Histone H10 antibody
    • Histone H5 antibody
    • MGC5241 antibody
    • N-terminally processed antibody
    see all

Anti-Histone H1.0 antibody [34] images

  • ab11079 staining Histone H1.0 in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab11079 at 10µg/ml and ab6046 (anti beta Tubulin) at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Mouse secondary (ab150117) at 2 μg/ml (shown in green) and AlexaFluor®594 Goat anti-Rabbit secondary (ab150084) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls: 1– Rabbit primary antibody and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.



  • Predicted band size : 20 kDa

    Western blot using ab11079 on HeLa whole cell extract (30 µg/lane).

    Exposure 5 sec using ECL.
    12.5% SDS-PAGE gel.

  • Overlay histogram showing HeLa cells stained with ab11079 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  • IHC image of ab11079 staining in human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11079, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


  • Predicted band size : 20 kDa

    Dr Albert Jordan, Center for Genomic Regulation (CRG), Spain.

    Mouse monoclonal to Histone H1.0 against recombinant human H1 isoforms H1.0 to H1.5 as depicted in the figure.

    Dilution 1/1000.

    Recombinant H1 isoforms courtesy of Dr Nicole Happel.

    (see attached review)

References for Anti-Histone H1.0 antibody [34] (ab11079)

This product has been referenced in:
  • Büchner A  et al. Sumoylation of p35 modulates p35/cyclin-dependent kinase (Cdk) 5 complex activity. Neuromolecular Med 17:12-23 (2015). Read more (PubMed: 25391294) »
  • Okami N  et al. Prevention of JNK phosphorylation as a mechanism for rosiglitazone in neuroprotection after transient cerebral ischemia: activation of dual specificity phosphatase. J Cereb Blood Flow Metab : (2012). WB . Read more (PubMed: 23032483) »

See all 15 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 2 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MRC 5VA - Lung Fibroblasts)
Loading amount 15 µg
Specification MRC 5VA - Lung Fibroblasts
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Mar 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"